![]() ![]() All vectors are available from the nonprofit Addgene repository here or upon request if necessary. A complete description of the vector design and evaluation is published here. utilize Gateway recombination cloning technology to enable high-throughput assembly (some vectors are also available in restriction cloning variants). incorporate insulator sequences and an artificial minimal promoter to ensure specific and uniform transgene expression, 3. phiC31 targeted transgene integration, 2. We have designed and developed a modular Drosophila vector series, pBID, to optimize and accelerate the production and analysis of Drosophila transgenes. The ability to reproducibly target expression of transgenes to defined subsets of cells is a critical experimental tool for understanding many biological processes. ![]()
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